abm | Immortalized Human Telomerized Endometrial Stromal Cells (SHT290) | T0739

abm | Immortalized Human Telomerized Endometrial Stromal Cells (SHT290) | T0739

Immortalization Method:

Immortalized with retroviral transduction containing hTERT

BioSafety Level:

II

Organism:

Human (H. sapiens)

Species:

Human

Source Organ:

Reproductive

Organ Type:

Reproductive

Growth Properties:

Adherent

Morphology:

Uniform bipolar, fibroblast-like shape

Passage Number:

N/A

Population Doubling:

4 - 6 days

Seeding Density:

N/A

Markers:

Puromycin, prolactin, plasminofen inhibitor type-1, vimentin, cytokeratin 13, cytokeratin 18

Donor Age:

Not disclosed

Donor Gender:

Female

Donor Ethnicity:

Not disclosed

Propagation:

Note: Initial seeding and subculturing must done at high density or the cells will cease to grow. Cells should be grown in the absence of puromycin.
The base medium for this cell line is Prigrow III medium available at abm. , and, Penicillin/Streptomycin Solution .
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.
To adapt for co-culture with epithelial cells: The base medium is a 1:1 mixture of Ham’s F12 (Gibco, Invitrogen Corp., Carlsbad, CA) and M199 basic medium (Sigma, St. Louis, MO) supplemented with 5% bovine calf serum (BCS; Hyclone, Logan, UT), 0.1% Mitoplus (BD Biosciences, Becton Dickinson, Franklin Lakes, NJ), 2 µg/ml of insulin (Sigma) and antibiotic/antimycotic solution diluted to yield 100 units/ml penicillin G sodium, 100 mg/ml streptomycin sulfate and 250 ng/ml amphotericin B (ABAM; Gibco).
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Decidualization procedure: The base medium for this procedure is Prigrow III medium available at abm. To make the complete growth medium, add fetal bovine serum to a final concentration of 2%, 1X (2mM)L-glutamine , 20 ng/ml human EGF and, Penicillin/Streptomycin Solution .
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Epifluorescence localization of cytoskeletal proteins conducted with mouse monoclonal antibodies against vimentin, cytokeratin 13, and cytokeratin 18

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C