abm | Immortalized Human Tonsillar Epithelial Cells (HTE) | T0721

abm | Immortalized Human Tonsillar Epithelial Cells (HTE) | T0721

Immortalization Method:

HPV16 infection

BioSafety Level:

II

Organism:

Human (H. sapiens)

Species:

Human

Source Organ:

Oral

Organ Type:

Oral

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

E7 and E1^E4 expression, G418-resistant

Donor Age:

Not disclosed

Donor Gender:

Not disclosed

Donor Ethnicity:

Not disclosed

Propagation:

The base medium for this cell line is Prigrow X medium available at abm. TM0721. Add 200 µg/ml G418 for selection pressure.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Quality Control:

1) Quantification of viral transcripts

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C