abm | Immortalized Human Umbilical Vein Endothelial Cells (HUV-1) | T0778

abm | Immortalized Human Umbilical Vein Endothelial Cells (HUV-1) | T0778

Immortalization Method:

Immortalized via introduction of cytoplasmic content (non-DNA) of tumor cells into primary cells

BioSafety Level:

II

Organism:

Human (H. sapiens)

Species:

Human

Source Organ:

Reproductive

Organ Type:

Reproductive

Growth Properties:

Adherent

Morphology:

Cobblestone

Passage Number:

N/A

Population Doubling:

24 - 30 hours

Seeding Density:

20,000 - 40,000 cells/cm²

Markers:

CD 31, VWF VIII

Donor Age:

Not disclosed

Donor Gender:

Not disclosed

Donor Ethnicity:

Not disclosed

Propagation:

Use of Gelatin Coating Solution is required for cell adhesion to the culture vessels. Grow cells in gelatin-coated culture vessels with the following conditions.
The base medium for this cell line is Prigrow IV medium available at abm. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%, Penicillin/Streptomycin Solution to a final concentration of 1%, Recombinant Human FGF2 to a final concentration of 10 ng/ml, Recombinant Human GH1 to a final concentration of 10 ng/ml, and Recombinant Human HGF to a final concentration of 10 ng/ml.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) IF; 2) IHS

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C