abm | Immortalized Human Umbilical Vein Endothelial Cells (PDL8 HUVEC) | T0725

abm | Immortalized Human Umbilical Vein Endothelial Cells (PDL8 HUVEC) | T0725

Immortalization Method:

Spontaneous

BioSafety Level:

II

Organism:

Human (H. sapiens)

Species:

Human

Source Organ:

Reproductive

Organ Type:

Reproductive

Growth Properties:

Adherent

Morphology:

Round

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

miR-216a expression

Donor Age:

Not disclosed

Donor Gender:

Not disclosed

Donor Ethnicity:

Not disclosed

Propagation:

The base medium for this cell line is Endothelial Cell Medium (ScienCell, San Diego, CA, USA). To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Assays for proliferation, migration, angiogenic and cell adhesion activity; 2) mRNA expression analysis; 3) Telomerase enzyme activity and telomere length measurement; 4) Western blot

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C