abm | Immortalized Human Umbilical Vein Endothelial Cells (PDL8 HUVEC) | T0725

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SKU:
T0725
Availability:
5 to 7 Days Shipment
Size:
1x10⁶ cells / 1.0 ml
zł7,303.61

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Description

abm | Immortalized Human Umbilical Vein Endothelial Cells (PDL8 HUVEC) | T0725

Immortalization Method:

Spontaneous

BioSafety Level:

II

Organism:

Human (H. sapiens)

Species:

Human

Source Organ:

Reproductive

Organ Type:

Reproductive

Growth Properties:

Adherent

Morphology:

Round

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

miR-216a expression

Donor Age:

Not disclosed

Donor Gender:

Not disclosed

Donor Ethnicity:

Not disclosed

Propagation:

Use of PriCoatTM T25 Flasks or Applied Cell Extracellular Matrix is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.

The base medium for this cell line is Endothelial Cell Medium (ScienCell, San Diego, CA, USA). To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Assays for proliferation, migration, angiogenic and cell adhesion activity; 2) mRNA expression analysis; 3) Telomerase enzyme activity and telomere length measurement; 4) Western blot

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C

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