abm | Immortalized Human Vascular Endothelial Cells (EA.hy926) | T0735

abm | Immortalized Human Vascular Endothelial Cells (EA.hy926) | T0735

Immortalization Method:

Spontaneous

BioSafety Level:

II

Organism:

Human

Species:

Human

Source Organ:

Human Umbilical Vein Endothelial Cells

Organ Type:

Human Umbilical Vein Endothelial Cells

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

von Willebrand factor (vWF), GMP-140, prostacyclin, platelet activating factor, tissue plasminogen activator, plasminogen activator inhibitor type I, thrombomodulin, vitronectin receptor

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 10%, 100 µM hypoxanthine, 0.4 µM aminopterin, and 16 µ;M thymidine (HAT), and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Quality Control:

1) ELISA for vWR

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C