abm | Immortalized Bcl-x Mouse Embryonic Fibroblasts - SV40T | T0749

abm | Immortalized Bcl-x Mouse Embryonic Fibroblasts - SV40T | T0749

Immortalization Method:

Transfection with pCDNA3 plasmid encoding SV40 large T antigen

BioSafety Level:

II

Organism:

Mouse

Species:

Mus musculus

Source Organ:

Embryo

Organ Type:

Embryo

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

20 - 30 hours

Seeding Density:

20, 000 - 50, 000 cells/cm10²

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C