abm | Immortalized C57BL6 Mouse Schwann Cells (1970C3) | T0769

abm | Immortalized C57BL6 Mouse Schwann Cells (1970C3) | T0769

Immortalization Method:

Spontaneous

BioSafety Level:

II

Organism:

Mus musculus (C57BL6)

Species:

Mouse

Source Organ:

Dorsal root ganglia neurons

Organ Type:

Brain

Growth Properties:

Adherent

Morphology:

Bipolar/spindle-shaped

Passage Number:

N/A

Population Doubling:

53 - 63 hours

Seeding Density:

30,000 - 50,000 cells/cm10²

Markers:

S100, laminin, p75NTR, NCAM, vimentin

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 5%, Recombinant Human NRG1 to a final concentration of 20 ng/mL, and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Immunofluorescence

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C