abm | Immortalized Mouse Atrioventricular Cushion Mesenchymal Cells (tsA58-AVM) | T0670

abm | Immortalized Mouse Atrioventricular Cushion Mesenchymal Cells (tsA58-AVM) | T0670

Sema6D gene.

Immortalization Method:

Conditional immortalization achieved in subsequent cultures as temperature sensitive SV40T antigen mutant gene (tsA58) expression driven by the c-interferon inducible promoter of the H-2Kb gene.

BioSafety Level:

II

Organism:

Mouse

Species:

Mouse

Source Organ:

Heart

Organ Type:

Heart

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

cTnt, MHC, PECAM, NFACT1, SMA, Vimentin

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10%, 1% L-glutamine , Penicillin/Streptomycin Solution to a final concentration of 1%, and 10 units/ml INFG. To make the complete INFG solution, dissolve 20 ug of INFG from Peprotech INC (Cat# 315-05) in 20ul PBS to get 1 mg/ml (10⁷ units/ml). Take 5 ul and add to into 10 ml of Prigrow II from abm with 10% Fetal Bovine Serum to acquire 500x stock (5x10³ units/ml).
Carbon dioxide (CO₂): 5%, Temperature: 33.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Immunostaining analysis; 2) Microarray analysis; 3) RT-PCR analysis;

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C