abm | Immortalized Mouse Cortical Collecting Duct Cell Line (mCCD(cl1)) | T0620

abm | Immortalized Mouse Cortical Collecting Duct Cell Line (mCCD(cl1)) | T0620

Immortalization Method:

Spontaneously immortalization

BioSafety Level:

II

Organism:

Mus musculus

Species:

Mouse

Source Organ:

Kidney

Organ Type:

Renal

Growth Properties:

Adherent

Morphology:

Other

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

In early passages, the cells do not form domes and grow more slowly. Split low passage cells at 1:6 ratio. At higher passages (above p15), the cells grow more rapidly and are recommended to be split at 1:10 ratio.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available at abm . To make the complete growth medium, add the following components to the base medium: 2% fetal calf serum, 0.87 µM insulin, 5 µg/ml human apotransferrin, 10 ng/ml EGF, 1 nM T3, 30 nM dexamethasone and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Quality Control:

1) Immunofluorescence and RT-PCR were used to confirm the expression of epithelial sodium channel (ENaC) and Na-K-ATPase; 2) RT-PCR was used to detect expression of MR, GR, and 11β-HSD2 ; 3) sodium transport response assayed through short-circuit current (electrophysiologic studies) and binding assays.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C