abm | Immortalized Mouse Embryo Pericytes | T0818

abm | Immortalized Mouse Embryo Pericytes | T0818

Immortalization Method:

Immortalized with retrovirus containing SV40 large T antigen

BioSafety Level:

II

Organism:

Mouse (M. musculus)

Species:

Mouse

Source Organ:

Embryo

Organ Type:

Embryo

Growth Properties:

Adherent

Morphology:

Mesenchymal stem cell-like

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

Ng2 (Cspg4), Cd146 (Mcam), Pdgfrb Neomycin resistance

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) PCR; 2) FACS

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C