abm | Immortalized Mouse Embryonic Epicardial Cells (MEC1) | T0671

abm | Immortalized Mouse Embryonic Epicardial Cells (MEC1) | T0671

Immortalization Method:

Spontaneous

BioSafety Level:

II

Organism:

Mus musculus

Species:

Mouse

Source Organ:

Heart

Organ Type:

Heart

Growth Properties:

Adherent

Morphology:

Cobblestone

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

Epicardin (Tcf21), Tbx18, and keratin 18

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium, available at abm. to a final concentration of 15% and Penicillin/Streptomycin Solution to a final concentration of 1%. Cells must be grown on gelatin coated plates. Gelatin Coating Solution (0.1%) is available at abm. .
Change media every 2-3 days.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Immunofluorescence; 2) Western blot; 3) qRT-PCR

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C