abm | Immortalized Mouse Floxed Fam20c Dental Papillar Mesenchymal Cells | T0039

abm | Immortalized Mouse Floxed Fam20c Dental Papillar Mesenchymal Cells | T0039

in vitro for research on the field of mineralization disorders.

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level:

II

Organism:

Postnatal 4-day Fam20c ^f/f Mice

Species:

Mouse

Source Organ:

First molars

Organ Type:

Skeletal Muscle

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow VIII medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Immunohistochemistry for gene expression associated with the differentiation and mineralization of odontoblasts

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C