abm | Immortalized Mouse Hepatic Sinusoidal Endothelial Cells - SV40 | T0102

abm | Immortalized Mouse Hepatic Sinusoidal Endothelial Cells - SV40 | T0102

Immortalization Method:

Serial passaging and transduction with pantropic recombinant retroviruses carrying SV40 Large T antigen

BioSafety Level:

II

Organism:

Mus musculus

Species:

Mouse

Source Organ:

Liver

Organ Type:

Liver

Growth Properties:

Adherent

Morphology:

Cobble-stone

Passage Number:

N/A

Population Doubling:

22-32 hours

Seeding Density:

5,000-7,000 cells/cm10²; Recommended split ratio is 1:2 to 1:3

Markers:

vWF and caveolin-1

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow I medium available at abm . To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%, Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1. Real Time PCR was used to quantify SV40 gene expression in immortalized cell line. 2. Western blot was used to confirm the presence of endothelial markers such as vWF and caveolin-1.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C