Description
abm | Immortalized Mouse Hexb-/- Schwann Cells (1113C1) | T0743
Immortalization Method:
Spontaneous immortalization
BioSafety Level:
II
Organism:
Mus musculus
Species:
Mouse
Source Organ:
Brain
Organ Type:
Brain
Growth Properties:
Adherent
Morphology:
Spindle-shaped
Passage Number:
N/A
Population Doubling:
N/A
Seeding Density:
N/A
Markers:
S100, laminin, p75NTR, GFAP, GM2 ganglioside
Donor Age:
N/A
Donor Gender:
N/A
Donor Ethnicity:
N/A
Propagation:
The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 5% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control:
1) RT-PCR; 2) Immunocytochemical analysis; 3) Enzyme assays and protein determination
Shipping Condition:
Dry Ice
Storage Condition:
liquid nitrogen or -180C