abm | Immortalized Mouse Neuronal Cells (M4b) | T0674

abm | Immortalized Mouse Neuronal Cells (M4b) | T0674

2+] response after neurotransmitter stimulation was rapid and transient as expected. The Immortalized Moue Neuronal Cells (M4b) is useful in studies of neuronal cell biology, viral infections, and neuronal cytotoxicity. The Immortalized Moue Neuronal Cells (M4b) can also be used as a control for the Immortalized Trisomy 16 Mouse Neuronal Cells (MTh) (Cat. T0675), which is a mouse model of Down Syndrome.

Immortalization Method:

Supplementation of plating medium with UCHT1 rat thyroid cell line-conditioned medium

BioSafety Level:

II

Organism:

Mouse

Species:

Mouse

Source Organ:

Spinal Cord

Organ Type:

Brain

Growth Properties:

Adherent

Morphology:

Neuronal

Passage Number:

N/A

Population Doubling:

14-24 hours

Seeding Density:

40,000 cells/cm10²

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10%, Bovine Serum (Sigma) to a final concentration of 10%, 1% L-glutamine and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) RT-PCR for SOD-1 and GAPDH; 2) Immunohistochemistry of neuronal markers; 3) Intracellular Ca²⁺ measurements;

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C