abm | Immortalized Mouse NPCNIH Schwann Cells (573C10) | T0745

abm | Immortalized Mouse NPCNIH Schwann Cells (573C10) | T0745

nih/npc^nih mice. NPC1 is a gene responsible for the majority of Niemann–Pick disease type C (NPC) patients. NPC1 protein was not detectable in homozygous 573C10 cell. 573C10 Schwann cell line can be useful to investigate the pathomechanism of nervous system lesions in NPC, the therapeutic approaches against neurological deficits in patients with NPC and, more in general, the biology of cholesterol, sphingolipid and fatty acid transports in the nervous system.

Immortalization Method:

Spontaneous immortalization

BioSafety Level:

II

Organism:

Mus musculus

Species:

Mouse

Source Organ:

Brain

Organ Type:

Brain

Growth Properties:

Adherent

Morphology:

N/A

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

S100, laminin, p75NTR, GFAP, GM2 ganglioside, filipin cholesterol cytochemistry

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 5% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Cytological analysis; 2) RT-PCR; 3) Western blot

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C