abm | Immortalized Mouse Olfactory Bulb Cells (OBC1) | T0235

abm | Immortalized Mouse Olfactory Bulb Cells (OBC1) | T0235

Immortalized Mouse Olfactory Bulb Cells (OBC1) is immortalized with non-replicating retrovirus carrying the avian Myc gene. The cells exhibit NF (neurofilament) and GalC (a marker for oligodendroglial lineage) markers. OBC1 cells have been shown to form tumors in animal models, hence has the ability to be tumorigenic. It is a valuable in vitro tool for studies relating to the functions and the biology of the olfactory bulb.

Immortalization Method:

Infection with retrovirus containing the avian Myc gene (pneoMLV)

BioSafety Level:

II

Organism:

New born CD-1xC57BL/6 mice

Species:

Mouse

Source Organ:

Olfactory bulbs

Organ Type:

Brain

Growth Properties:

Adherent

Morphology:

Flattened|Dendritic processes

Passage Number:

N/A

Population Doubling:

48 hours

Seeding Density:

20,000 cells/cm10²

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10%, 0.3 mg/ml G418 (G271), and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Resistance to 0.3 mg/ml G418, 2) Southern or Northern blot analysis of the viral integration site, 3) immunocytochemical analysis for markers

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C