abm | Immortalized Mouse Olfactory Bulb Cells (OBC13) | T0237

abm | Immortalized Mouse Olfactory Bulb Cells (OBC13) | T0237

Immortalized Mouse Olfactory Bulb Cells (OBC13) is immortalized with non-replicating retrovirus carrying the avian Myc gene. It is a sister derivative from the Immortalized Mouse Olfactory Bulb Cells (OBC1) (T0235). The cells exhibit GalC (a marker for oligodendroglial lineage) marker, but has the absence of NF (neurofilament marker). Voltage-dependent potassium channels are expressed as well. OBC13 cells have been shown to form tumors in animal models, hence has the ability to be tumorigenic. It is a valuable in vitro tool for studies relating to the functions and the biology of the olfactory bulb.

Immortalization Method:

Infection with retrovirus containing the avian Myc gene (pneoMLV)

BioSafety Level:

II

Organism:

New born CD-1xC57BL/6 mice

Species:

Mouse

Source Organ:

Olfactory bulbs

Organ Type:

Brain

Growth Properties:

Adherent

Morphology:

Flattened|Dendritic processes

Passage Number:

N/A

Population Doubling:

23-33 hours

Seeding Density:

2,500 - 20, 000 cells/cm10²

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

Grow cells in Poly-L-Lysine (TM061)-coated flasks with the following conditions. The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10%, 0.3 mg/ml G418 (G271), and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Resistance to 0.3 mg/ml G418, 2) Southern or Northern blot analysis of the viral integration site, 3) immunocytochemical analysis for markers

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C