Description
abm | Immortalized Mouse Olfactory Bulb Cells (OBL21) | T0238
Immortalization Method:
Infection with retrovirus containing the avian Myc gene (pneoMLV)
BioSafety Level:
II
Organism:
New born CD-1 mice
Species:
Mouse
Source Organ:
Olfactory bulbs
Organ Type:
Brain
Growth Properties:
Adherent
Morphology:
Round|Clusters
Passage Number:
N/A
Population Doubling:
N/A
Seeding Density:
Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers:
N/A
Donor Age:
N/A
Donor Gender:
N/A
Donor Ethnicity:
N/A
Propagation:
Grow cells in Poly-L-Lysine (TM061)-coated flasks with the following conditions. The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10%, 0.3 mg/ml G418 (G271), and Penicillin/Streptomycin Solution to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control:
1) Resistance to 0.3 mg/ml G418, 2) Southern or Northern blot analysis of the viral integration site, 3) immunocytochemical analysis for markers
Shipping Condition:
Dry Ice
Storage Condition:
liquid nitrogen or -180C