abm | Immortalized Mouse Preadipocyte Cells (ScAP-23) | T0544

abm | Immortalized Mouse Preadipocyte Cells (ScAP-23) | T0544

Immortalization Method:

Serial passaging and infection with retroviruses carrying hTERT gene

BioSafety Level:

II

Organism:

Mouse

Species:

Mouse

Source Organ:

Adipose tissue

Organ Type:

Adipose

Growth Properties:

Adherent

Morphology:

Fibroblast-like

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

Grow cells in T25 ECM-coated flasks (G299) with the following conditions. The base medium for this cell line is Prigrow III medium . To make the completed growth medium, add the following components to the base medium at a final concentration of fetal bovine serum to a final concentration of 10%, 2mM L-glutamine , and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Oil Red O staining at day 7 after inducing adipocyte differentiation to visualize lipid droplets; 2) Harvest RNA after induction of adipogenesis and perform RT-PCR and Northern Blot to detect transcript level of adipocyte markers SCD1, aFABP, ATGL, FAS, LDL, and GPDH B.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C