abm | Immortalized Mouse Promyelocyte Cells (MPRO C.2) - SV40 | T0686

abm | Immortalized Mouse Promyelocyte Cells (MPRO C.2) - SV40 | T0686

Immortalization Method:

Immortalized with retroviral vector LRARα403SN

BioSafety Level:

II

Organism:

Mus musculus

Species:

Mouse

Source Organ:

Bone Marrow

Organ Type:

Bone Marrow

Growth Properties:

Suspension

Morphology:

Promyelocyte characteristics; tend to form aggregates

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

Express high levels of retroviral mRNA harboring the truncated RARα403 sequence; mouse neutrophil-specific antigen 7/4; positive staining for chloroacetate esterase

Donor Age:

Six weeks old

Donor Gender:

Male

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow V medium available at abm. to a final concentration of 20%, Penicillin/Streptomycin Solution to a final concentration of 1%, WEHI-3B conditioned medium to a final concentration of 20%, Polybrene to a final concentration of 4 µg/ml, Recombinant Mouse GM-CSF (CSF2) to a final concentration of 2.5 ng/ml, Recombinant Human IL1B to a final concentration of 10 ng/ml, and Recombinant Human IL6 to a final concentration of 20 ng/ml.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated serum for cell culture unless specified otherwise.

Quality Control:

1) Growth pattern analysis; 2) Northern analysis; 3) Transient expression analysis

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C