abm | Immortalized Mouse Sertoli Cells (ST38c) | T0652

abm | Immortalized Mouse Sertoli Cells (ST38c) | T0652

Immortalization Method:

Isolated from a transgenic mouse carrying SV40 large T Antigen under the control of the human vimentin promoter

BioSafety Level:

II

Organism:

Mouse

Species:

Mouse

Source Organ:

Testes

Organ Type:

Reproductive

Growth Properties:

Adherent

Morphology:

Spindle shaped

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

Male

Donor Ethnicity:

N/A

Propagation:

ST38c cells must be grown in Prigrow III base medium supplemented with a final concentration of fetal bovine serum to a final concentration of 5% and a final concentration of Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1. Androgen receptor mRNA was confirmed via RT-PCR while western blot confirmed AR protein expression.

2. Androgen receptor regulated genes were identified via microarray assays.

3. SV40 Tag expression confirmed through fluorescence microscopy

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C