abm | Immortalized Mouse Smooth Muscle/Myometrial-Like Cells (SMU1-10) | T0651

abm | Immortalized Mouse Smooth Muscle/Myometrial-Like Cells (SMU1-10) | T0651

Immortalization Method:

Isolated from a H-2Kb-tsA58 transgenic mouse carrying a thermolabile SV40 large T-antigen gene

BioSafety Level:

II

Organism:

H-2K^b-tsA58 transgenic Mouse

Species:

Mouse

Source Organ:

Uterus

Organ Type:

Reproductive

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

26 hours

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

SM-MHC, SM22 alpha, connexin 43, thromboxane A2 receptor, progesterone receptor

Donor Age:

N/A

Donor Gender:

Female

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10%, 10 U/ml IFN-γ, and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Indirect immunofluorescence, Northern blot, and RT-PCR were utilized for smooth muscle-related markers

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C