abm | Immortalized Mouse Urogenital Sinus Mesenchymal Cells (UGSM-2) | T0653

abm | Immortalized Mouse Urogenital Sinus Mesenchymal Cells (UGSM-2) | T0653

Co-culture of UGSM-2 cells with human prostate epithelium cells BPH-1, or co-grafting in vivo results in organized clusters of BPH-1 cells surrounded by a mantle of the UGSM-2 cells. The cells are responsive to Sonic hedgehog (SHH), an important signaling factor in prostate development, and mimic the transcriptional response of the intact UGS mesenchyme. This cell line is a useful model for studying the prostate stroma-epithelium signalling way, which plays an important role in prostate development and cancer progression.

Immortalization Method:

p16^INK4a and p19^ARF Knockout

BioSafety Level:

II

Organism:

Mouse

Species:

Mouse

Source Organ:

Prostate

Organ Type:

Reproductive

Growth Properties:

Adherent

Morphology:

Spindle shaped|Fibroblast-like

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available at abm. to a final concentration of 10%, Gentamicin (50ug/ml) + 1% ITS + Glutamine (1x) + 10^-8M DHT, and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Real Time PCR was used to shows activation of SHH target genes

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C