abm | Immortalized Wildtype Mouse Muscular Dystrophy Myoblast Cells - SV40 | T0646

abm | Immortalized Wildtype Mouse Muscular Dystrophy Myoblast Cells - SV40 | T0646

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying SV40

BioSafety Level:

II

Organism:

Mus musculus

Species:

Mouse

Source Organ:

Muscle

Organ Type:

Skeletal Muscle

Growth Properties:

Adherent

Morphology:

Elongated

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

Use of Matrigel Matrix is required for cell adhesion to the culture vessels. Grow cells in Matrigel-coated culture vessels with the following conditions.
The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 10%, Penicillin/Streptomycin Solution to a final concentration of 1%, L-glutamine to a final concentration of 1%, and Chick Embryo Extract (CEE) to a final concentration of 0.5%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated serum for cell culture unless specified otherwise.

Quality Control:

Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C