abm | Immortalized Porcine Alveolar Macrophage Cells (PAM-KNU) - hTERT | T0741

abm | Immortalized Porcine Alveolar Macrophage Cells (PAM-KNU) - hTERT | T0741

Immortalization Method:

Immortalized with retrovirus containing hTERT (pFB-Neo-hTERT)

BioSafety Level:

II

Organism:

Pig

Species:

Porcine

Source Organ:

Lung

Organ Type:

Airway/Lung

Growth Properties:

Adherent

Morphology:

Macrophage-like

Passage Number:

N/A

Population Doubling:

24-36 hours

Seeding Density:

N/A

Markers:

CD163

Donor Age:

8 weeks

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow II medium available at abm. to a final concentration of 5%, 20 µl/ml Dexamethasone (Sigma), and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Recommended Split Ratio: 1:5.

Quality Control:

1. hTERT was determined via RT-PCR.

2. Presence of CD163 detected by FACS.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C