abm | Immortalized Rat Conjunctival Epithelial Cells (CJ4.3) - SV40 | T0369

abm | Immortalized Rat Conjunctival Epithelial Cells (CJ4.3) - SV40 | T0369

Immortalization Method:

Immortalization by transfection with SV40 large T antigen

BioSafety Level:

II

Organism:

Fischer

Species:

Rat

Source Organ:

Eye

Organ Type:

Eye

Growth Properties:

Adherent

Morphology:

Epithelial-like

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

Cytokeratin 4, Cytokeratin 7

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available at abm . To make the completed growth medium, add the following components to the base medium: BSA to final concentration of 0.5%, 10 mM HEPES, 1X trace elements B, 10 μg/ml insulin, 5.5 μg/ml transferrin, 50 ng/ml sodium selenate, 20 μg/ml ethanolamine, 0.3 μg/ml glucagon, 0.1 μM dexamethasone, 0.1 μM retinoic acid, 0.3 μM 3, 3’, 5 triiodo-L thyronine, 80 ng/ml epidermal growth factor, 50 μg/ml gentamycin sulfate (Invitrogen-GIBCO) and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Quality Control:

1) Immunofluorescence confocal microscopy demonstrating cytokeratin expression

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C