abm | Immortalized Rat Neural Stem Cells (1464R) | T0746

abm | Immortalized Rat Neural Stem Cells (1464R) | T0746

Immortalization Method:

Spontaneous immortalization

BioSafety Level:

II

Organism:

Rattus norvegicus (Fischer 344)

Species:

Rat

Source Organ:

Brain

Organ Type:

Brain

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

N/A

Donor Age:

12 weeks

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

Cultures are grown on poly(2-hydroxyethylmethacrylate) (PHEMA; Sigma) coated cell culture vessels.
The base medium for this cell line is Neurobasal medium containing 2 mM L-glutamine, 2% B27 supplement (Thermo Fisher Scientific, Waltham, CA, USA), 10 ng/mL of FGF2 , 10 ng/mL of EGF , and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

To differentiate into neuronal and glial cells:

Quality Control:

N/A

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C