abm | Immortalized Rat Olfactory Ensheathing Glia SV40 | T0249

abm | Immortalized Rat Olfactory Ensheathing Glia SV40 | T0249

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen (Plasmid pEF321-T, with EF1? promoter)

BioSafety Level:

II

Organism:

Rat (P-21)

Species:

Rat

Source Organ:

Nerve fiber layer of rat oflactory bulbs

Organ Type:

Brain

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

p75-NGFr, Nestin, GFAP, Sulfatide O4

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available at abm . To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%, pituitary extract to 20ug/ml, forskolin to 2uM and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Immunohistochemical staining of SV40 T antigen

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C