abm | Immortalized Rat Ovarian Granulosa Cells (RGA-1) | T0758

abm | Immortalized Rat Ovarian Granulosa Cells (RGA-1) | T0758

Immortalization Method:

Immortalized with a temperature-sensitive mutant (tsA209) of SV40

BioSafety Level:

II

Organism:

Rat (R. norvegicus)

Species:

Rat

Source Organ:

Ovary

Organ Type:

Ovary

Growth Properties:

Adherent/Suspension

Morphology:

Elongated, overlapped and binucleated at 33°C or is mononucleated at 39°C.

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

PCNA

Donor Age:

21 days old

Donor Gender:

Female

Donor Ethnicity:

Not disclosed

Propagation:

The base medium for this cell line is Prigrow II medium available at abm. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 33.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Western blot; 2) Northern blot; 3) Immunofluorescence

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C