abm | Immortalized Rat Schwann Cells (IFRS1) | T0294

abm | Immortalized Rat Schwann Cells (IFRS1) | T0294

Immortalization Method:

Spontaneous Immortalization

BioSafety Level:

II

Organism:

Fischer 344 Rat

Species:

Rat

Source Organ:

Brain

Organ Type:

Brain

Growth Properties:

Adherent

Morphology:

Spindle shaped

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10%, 20 ng/ml neuregulin-beta, 2 µM forskolin/DMSO, and Penicillin/Streptomycin Solution to a final concentration of 1%
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Quality Control:

Immunoreactivity to cell markers

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C