abm | Immortalized Rat Suprachiasmatic Nucleus Cells (SCN2.2) | T0296

abm | Immortalized Rat Suprachiasmatic Nucleus Cells (SCN2.2) | T0296

Immortalization Method:

Serial passaging and transduction with retrovirus carrying adenovirus 12S E1A gene

BioSafety Level:

II

Organism:

Long-Evans Rat, fetal

Species:

Rat

Source Organ:

Brain

Organ Type:

Brain

Growth Properties:

Adherent

Morphology:

Round|Flattened

Passage Number:

N/A

Population Doubling:

17-27 hours

Seeding Density:

10,000 cells/cm10²

Markers:

NSE, PGP, and MAP-2

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

Grow cells in poly-D-lysine or laminin-coated plates. The base medium for this cell line is Prigrow VII, available at abm Cat. No. to a final concentration of 10%, 2 µg/ml glucose, 2 mM L-glutamine, and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Immunofluorescent staining and western blot analysis for E1A; 2) Immunocytochemical screening with cell type-specific antigens to distinguish phenotypes

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C