abm | NonO Stable Knockdown Jurkat Cell Line | T6124

abm | NonO Stable Knockdown Jurkat Cell Line | T6124

NonO (Non-POU domain-containing octamer-binding) protein is called p54nrb (nuclear RNA binbing) is encoded by the NONO gene. It has been associated with melanoma, and has strongh expression in melanoma tissue samples. NonO-specific shRNA-expressing GFP lentiviral vector pGIPZ was used to knockdown NonO expression in Jurkat cells to generate the NonO Stable Knockdown Jurkat Cell Line to study the effects and role of NonO and cancer.

Biosafety:

II

Organism:

Human

Source Organ:

Blood

Growth Properties:

Suspension

Morphology:

Round

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

100,000 cells/ml

Markers:

Puromycin-resistance

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow II medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 5% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) To determine knockdown of NonO, anti-NonO antibody (abcam, ab13164) was used for immunoblotting.

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C