abm | SIRT7 Stable Knockdown HeLa Cell Line | T6164

abm | SIRT7 Stable Knockdown HeLa Cell Line | T6164

SIRT7 Stable Knockdown HeLa Cell Line was generated by lentiviral driven expression of shRNAs. Lack of Sirt7 is associated with reduced recruitment of DNMT1 and Sirt1 at rRNA genes leading to hyperacetylation of histones, reduced DNA methylation, fragmentation of the nucleolar structure and loss of rDNA repeats leading to an increased spontaneous immortalization of primary mouse embryonic fibroblasts.

Biosafety:

II

Organism:

Human

Source Organ:

Cervix

Growth Properties:

Adherent

Morphology:

Epithelial

Clones:

N/A

Passage Number:

N/A

Population Doner:

41 - 51 hours

Seeding Density:

10,000 - 20,000 cells/cm²

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III available at abm

Preservation:

N/A

Quality Control:

1) Immunoprecipitation; 2) Co-immunoprecipitation; 3) Western blot; 4) Chromatin immunoprecipitation; 5) Immunofluorescence analysis; 6) qPCR

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C