abm | Araf CRISPR Knocked out RAW264.7 Stable cell Cell Line (Mouse) T3-3 | T9529

abm | Araf CRISPR Knocked out RAW264.7 Stable cell Cell Line (Mouse) T3-3 | T9529

Araf Stable Knockout Mouse RAW264.7 Cell Line is established via CRISPR/Cas9 mediated frameshift mutation of the Araf gene (NM_009703) using the following guide RNA sequence: CCGTCTGCGTTGACATGAGT. Araf gene maybe involved in cell growth and development, this cell line is useful in the study of cancer treatment. This cell line is sequenced verified to be biallelic knockout.

Biosafety:

II

Organism:

Mouse

Source Organ:

Ascites

Growth Properties:

Adherent

Morphology:

Cobblestone

Clones:

Monoclone

Passage Number:

6

Population Doner:

N/A

Seeding Density:

N/A

Markers:

Puromycin

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available in ABM

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) 4.0 µg/ml of puromycin (G264) was used for clonal selection; 2) Araf gene knockout confirmed via surveryor assay and sanger sequencing of targeted genomic region

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C