abm | AT2 Knockout Immortalized Mouse Renal Proximal Tubule Cell Line | T0625

abm | AT2 Knockout Immortalized Mouse Renal Proximal Tubule Cell Line | T0625

The proximal tubule is part of the nephron duct system in the kidney, involved in regulating the renin-angiotension system. Cells of this region aid in regulating sodium and volume homeostasis as well as regulating the systemic blood pressure. The protein Angiotension II (Ang II) and its receptors AT₁ and AT₂ are some of the most studied proteins in this system. The AT2 Knockout Immortalized Mouse Renal Proximal Tubule Cell Line is a conditionally immortalized mouse renal proximal tubule cells isolated from the mouse harboring thermolabile mutation (tsA58) of the simian virus 40 large T antigen. The functional expression of the SV40 large T antigen is induced by culturing the cells in vitro in medium containing IFN γ at a temperature permissive (33°C). At a non-permissive temperature (37°C-39°C), the cells cease to proliferate. When paired up with the wild type

Biosafety:

II

Organism:

C57B/6 mouse with AT₂^-/- genotype

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Cobblestone

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

(1) RT-PCR was performed to examine products expressed in mutant AT2 -containing cells. (2) Immunostaining was performed to detect distribution of AT1and AT2, plus assess morphology. (3) Western blotting was performed to confirm presence of AT2. (4)Electrical conductivity and changes in short-circuit currents were measured to determine how similar immortalized cells were to their non-immortalized counterparts. (5)Radioimmunoassays were performed to detect guanylate cyclase levels as a measure of AT2 receptor functionality.

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C