abm | Bax-Bak Double Knockout Immortalized Mouse Kidney Epithelial Cell Line | T3026

abm | Bax-Bak Double Knockout Immortalized Mouse Kidney Epithelial Cell Line | T3026

Key regulators of apoptosis, such as members of the BCL-2 family, have long been linked to the development of cancer. The Bax/Bak Double Knockout Immortalized Mouse Kidney Epithelial Cells (bax^-/-/ bak^-/- iBMK) is derived from stable transformation of E1A and p53DD into primary baby kidney epithelial cells from mice deficient for Bak and Bax. Bax and Bak are functionally redundant downstream regulators to the mechanism of apoptosis, therefore this double knockdown cell line provides a valuable insight into pro-apoptotic proteins and programmed cell death. Together with the wild-type iBMK (T0082), Bax knockout iBMK (bax^-/- iBMK, T3024) and Bak Knockout iBMK (Bak^-/- iBMK, T3025), these cell lines are suitable in studying the relationships between BCL-2 family and cancer progression, prognosis and significance to treatment.

Biosafety:

II

Organism:

Baby C57B/6 mouse with bak^-/-, bax^-/- genotype

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Cuboidal

Clones:

D3

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

E-cadherin, CK8 and CK18

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm To make the complete growth medium, add the following components to the base medium: fetal bovine serum

Preservation:

N/A

Quality Control:

1. Western blot was used to confirm the presence of E1A and p53DD gene in the immortalized cell line. 2. Western blot was used to check epithelial markers E-cadherin and cytokeratin expression. 3. PCR genotyping of the pup tail was performed to verify the genotype of the cell line.

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C