abm | eNOS Stable Knockout Mouse Aortic Endothelial (iMAEC-eNOS) Cell Line | T3180

abm | eNOS Stable Knockout Mouse Aortic Endothelial (iMAEC-eNOS) Cell Line | T3180

The eNOS Stable Knockout Mouse Aortic Endothelial (iMAEC-eNOS) Cell Line was derived from primary mouse aortic endothelial cells isolated from the thoracic and abdominal aortas of eNOS knockout mice. The primary cells were immortalized using polyoma middle-sized T-antigen (PmT) method. The eNOS Stable Knockout Mouse Aortic Endothelial (iMAEC-eNOS) Cell Line is able to retain the properties and phenotype of the parental cells including the expression of common markers of endothelial cells including PECAM1, VE-cadherin, and von Willebrand Factor but it is not able to express the eNOS protein. Another interesting characteristic of this cell line is the elevated expression of VCAM-1. When tested for the functional responses to physiologically relevant shear stresses, such as laminar shear, the eNOS Stable Knockout Mouse Aortic Endothelial (iMAEC-eNOS) Cell Line still maintained the ability to form tubes as expected from primary endothelial cells. With these characteristics, the eNOS Stable Knockout Mouse Aortic Endothelial (iMAEC-eNOS) Cell Line is a useful model in cardiology, particularly in studying vascular biology and pathobiology in vitro and in studies focusing on eNOS.

Biosafety:

II

Organism:

Mouse

Source Organ:

Heart

Growth Properties:

Adherent

Morphology:

Elongated

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

N/A

Markers:

PECAM1, VE-cadherin, von Willebrand Factor

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

Isolated from genetically modified eNOS knockout mice

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is PriGrow VI medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) FACS; 2) Characterization by Dil-Ac-LDL staining and immunostaining

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C