abm | FRK Stable Knockout Human H1299 Cell Line | T9505

abm | FRK Stable Knockout Human H1299 Cell Line | T9505

FRK Stable Knockout Human H1299 Cell Line is established via CRISPR/Cas9 mediated frameshift mutation of the FRK gene (NM_002031) using the following guide RNA sequence: GAGGACTTGAGCTTCCGAGC. FRK gene has important functions during G1 and S phase of the cell cycle and thus can be used to study cell proliferation. This cell line is sequenced verified to be biallelic knockout.

Biosafety:

II

Organism:

Human

Source Organ:

Lung

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

1C10

Passage Number:

4

Population Doner:

N/A

Seeding Density:

N/A

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow II medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) 0.5 µg/ml of puromycin (G264) was used for clonal selection; 2) FRK gene knockout confirmed via surveryor assay and sanger sequencing of targeted genomic region

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C