abm | GALK2+GALE Stable Knockout HEK293T Cell Line | T6152

abm | GALK2+GALE Stable Knockout HEK293T Cell Line | T6152

The UDP-galactose-4 epimerase (encoded by GALE) and Galactokinase 2 (GALK2) are both major enzymes in the Leloir pathway, the primary pathway for galactose metabolism. UDP-galactose-4 epimerase modifies galactose and acetyl galactose, generating precursors for O-linked glycolsylation. Deficiencies in these genes can lead to Galactosemia: a condition that is caused by the accumulation of galactose in the blood and tissue of individuals that cannot properly metabolize the galactose in their diets. As this cell line is derived from a human source, it may be useful in the study of glycosylation and how it is affected during instances of disease.

Biosafety:

II

Organism:

Human

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Epithelial

Clones:

N/A

Passage Number:

N/A

Population Doner:

22 - 32 hours

Seeding Density:

20,000 - 40,000 cells/cm²

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

Embryo

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III available from abm

Preservation:

N/A

Quality Control:

1) Confirmed GALK 2 knockout through Western Blot analysis (Figure 5A) and sequencing (Figure S3). Tested O-glycosylation levels through enzymatic activity on the substrate SIVmac239 gp120 via Western Blot and SDS gel analysis (Figure 5B).

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C