abm | GALK2 Stable Knockout HEK293T Cell Line | T6154

abm | GALK2 Stable Knockout HEK293T Cell Line | T6154

The Galactokinase 2 (GALK2) is an efficient kinase of N-acetylgalactosamine in primary pathway for galactose metabolism the Leloir pathway that also acts as a galactokinase when galactose concentrations are high.

Biosafety:

II

Organism:

Human

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Epithelial

Clones:

N/A

Passage Number:

N/A

Population Doner:

33 - 43 hours

Seeding Density:

40,000 - 50,000 cells/cm²

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

Embryo

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III available from abm

Preservation:

N/A

Quality Control:

1) Confirmed GALK knockout through Western Blot analysis (Figure 5A) and sequencing (Figure S3). 2)Tested O-glycosylation levels through enzymatic activity on the substrate SIVmac239 gp120 via Western Blot and SDS gel analysis (Figure 5B).

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C