abm | NLK Stable Knockout Human A549 Cell Line | T9510

abm | NLK Stable Knockout Human A549 Cell Line | T9510

NLK Stable Knockout Human A549 Cell Line is established via CRISPR/Cas9 mediated frameshift mutation of the NLK gene (NM_016231) using the following guide RNA sequence: ACACCATCTTCATCCGGGGT. NLK gene has important implications for Protein Coding and this cell line is useful in the study of transferase activity. This cell line is sequenced verified to be biallelic knockout.

Biosafety:

II

Organism:

Human

Source Organ:

Lung

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

Monoclone

Passage Number:

6

Population Doner:

N/A

Seeding Density:

N/A

Markers:

Puromycin

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow II medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) 1.0 µg/ml of puromycin (G264) was used for clonal selection; 2) NLK gene knockout confirmed via surveryor assay and sanger sequencing of targeted genomic region

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C