abm | PARP-1 Knockout Immortalized Mouse Heart Endothelial (HYKO6) Cell Line | T3031

abm | PARP-1 Knockout Immortalized Mouse Heart Endothelial (HYKO6) Cell Line | T3031

Poly(ADP-ribose) polymerase 1 (PARP-1) is an ADP-ribosylating enzyme that plays a critical role in DNA damage repair, in addition to functioning as a context- specific regulator of transcription factors. Various pathophysiological conditions, such as reperfusion injury, endotoxic shock, diabetes and aging are associated with endothelial cell dysfunction mediated by PARP-1.

Biosafety:

II

Organism:

9-10 weeks old 129/Sv x C57BL/6 mouse with PARP-1^-/- genotype

Source Organ:

Heart

Growth Properties:

Adherent

Morphology:

Cobblestone

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

CD105, CD31, ICAM-2, VCAM-1, vWF

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow II medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Flow cytometry was used to confirm the expression of endothelial cell markers such as CD105, CD31, ICAM-2, VCAM-1 and MHC Class I antigens; 2) Immunofluorescence staining was used to assess the vWF expression; 3) Western blot was used to evaluate the expression of SV40 T-antigen in the immortalized cell line.

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C