abm | Screen It™ CRISPR Cas9 Cleavage Detection Kit| G990

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SKU:
G990
Availability:
1 to 2 weeks
Size:
100 Reactions
$1,419.73

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Description

abm | Screen It™ CRISPR Cas9 Cleavage Detection Kit| G990

Product Description and Application

abm’sScreen It™ CRISPR Cas9 Cleavage Detection Kitis a robust and precise RNA-dependent assay used to identify successful insertions and/or deletions (indels) of genomic DNA following a CRISPR-Cas9 experiment. CRISPR-Cas9 can introduce indels on either one or both copies of a given mammalian gene.abm’s Screen It™ CRISPR Cas9 Cleavage Detection Kit can easily identify if a given clone is monoallelic (has mutations in one copy), biallelic (both copies mutated) or is unchanged wild-type which is considered a major screening advantage over the standard T7E1 Surveyor Assay. This kit uses PCR to first amplify the sgRNA target site from CRISPR edited cells; this amplicon is subsequently cleaved by a ribonucleoprotein (RNP) complex which generates a unique pattern that can be easily resolved on agarose gel and interpreted by the user. Using this kit after gene editing saves time and energy from the arduous screening process and provides clear genotyping results. 

Storage Condition

Store all components at -20°C.

 

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1 Review

  • 4

    Efficiency

    Posted by Pr. Michael Thompson on 13th Jun 2024

    This Kit has been a game-changer in our gene editing experiments. Its sensitivity and ease of use have greatly expedited our research process, allowing us to quickly assess CRISPR/Cas9 cleavage efficiency with confidence. This kit is a must-have for any lab conducting gene editing studies

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