abm | α3 nAChR Stable Expressing HEK293 Cell Line | T6422

abm | α3 nAChR Stable Expressing HEK293 Cell Line | T6422

It has been shown that neurons in constant exposure to nicotine will experience an up-regulation in nicotinic acetylcholine receptor (nAChR) expression. To generate this stable cell line, nAChR was first transfected into the HEK293 cells, followed by the introduction of the rabbit polyclonal antibody α3 via the pBUD vector. These stable cells were selected for in zeocin-containing complete media.

Biosafety:

II

Organism:

Human (H. sapiens)

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

30 - 40 hours

Seeding Density:

30,000 - 50,000 cells/cm²

Markers:

pBudCE4.1 vector, zeocin resistance

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

Use of PriCoatTM T25 Flasks G299) or Applied Cell Extracellular Matrix G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.

Preservation:

1. Freeze Medium: 90% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.Freeze Medium: 90% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Western blot; 2) Immunocytochemistry

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C