abm | Akt1 Stable Expressing NIH3T3 Cell Line | T6333

abm | Akt1 Stable Expressing NIH3T3 Cell Line | T6333

NIH3T3 cells were transfected with the pLKO-AS3W-Puro-flag vector carrying the Akt1 wildtype gene. Puromycin selection was completed to establish the Akt1 Stable Expressing NIH3T3 Cell Line. The cells are a great tool to study Akt1, which is a serine/threonine kinase involved in processes such as, cell proliferation, survival and metabolism, and if hyperactivated is linked to cancer progression.

Biosafety:

II

Organism:

Mouse (M. musculus)

Source Organ:

Embryo

Growth Properties:

Adherent

Morphology:

Fibroblast

Clones:

N/A

Passage Number:

N/A

Population Doner:

24 - 34 hours

Seeding Density:

N/A

Markers:

Akt1, puromycin-resistant

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: 90% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Western blot

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C