abm | AXL Stable Ba-F3 Cell Line | T3064

abm | AXL Stable Ba-F3 Cell Line | T3064

Fusing a kinase domain to a homodimerization domain such as the PNT domain of ETV6 can result in activated kinase. The AXL Stable Ba/F3 Cell Line expresses activated AXL tyrosine kinase that is linked to a GFP reporter (GFP-ETV6-AXL in SED2 vector). Ba/F3 cells expressing activated tyrosine kinase become completely dependent on the kinase activity for survival and as a result, the cells can be propagated even in the absence of IL-3. Due to the fusion, kinase became autophosphorylated resulting in activated proliferation and survival pathways in the cells to be independent on IL-3.

Biosafety:

I

Organism:

Mouse

Source Organ:

Bone Marrow

Growth Properties:

Suspension

Morphology:

Round

Clones:

N/A

Passage Number:

N/A

Population Doner:

29 - 35 hours

Seeding Density:

120,000 cells/ml

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

AXL tyrosine kinase (GFP-ETV6-AXL) via retroviral transduction

Freeze Thaw:

If the cells are observed to be stressed, we recommend to seed cells into 1 T25 flask with 10-13ml of completed growth medium, and leave the flask standing upright for recovery.

Propagation:

The base medium for this cell line is Prigrow II medium available at abm

Preservation:

N/A

Quality Control:

WB

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C