abm | BRCA1 CRISPR Stable Knockout Human Fibroblast Cell Line | T6245

abm | BRCA1 CRISPR Stable Knockout Human Fibroblast Cell Line | T6245

BRCA1 is a tumor suppressor gene that plays a significant role in DNA repair pathways. Human fibroblast cells were transfected with CRISPR/Cas9 plasmids which target BRCA1 to generate the BRCA1 CRISPR Stable Knockout Human Fibroblast Cell Line. This cell line demonstrates that a deficiency in a single oncosuppressor gene can be enough to allow a malignant transformation when exposed to human cancer serum. In addition, this cell line illustrates that there may be a non-classical pathway to explain cancer trait exchange in malignant and non malignant cells.

Biosafety:

II

Organism:

Human

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Fibroblast-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

N/A

Markers:

CEA, CK20, CDX-2, CK7/CK19 and AE1/AE3

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available at abm

Preservation:

1. Freeze Medium: 90% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Western blot; 2) Immunohistochemistry; 3) Microscopy

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C