abm | BTK Stable Ba-F3 Cell Line | T3147

abm | BTK Stable Ba-F3 Cell Line | T3147

Fusing a kinase domain to a homodimerization domain such as the PNT domain of ETV6 can result in activated kinase. The BTK Stable Ba/F3 Cell Line expresses activated BTK tyrosine kinase that is linked to a GFP reporter (GFP-ETV6-BTK in MSCV-puro vector). Even though this cell line expresses activated tyrosine, it is non-transforming, meaning that the cells still require IL-3 to grow.

Biosafety:

I

Organism:

Mouse

Source Organ:

Bone Marrow

Growth Properties:

Suspension

Morphology:

Round

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

BTK tyrosine kinase (GFP-ETV6-BTK) via retroviral transduction

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow II medium available at abm

Preservation:

N/A

Quality Control:

WB

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C