abm | c-Kit N705A-N938A Stable Ba-F3 Cell Line | T3051

abm | c-Kit N705A-N938A Stable Ba-F3 Cell Line | T3051

c-Kit or stem cell factor (SCF) receptor is a type III tyrosine kinase receptor that has a major role in the hematopoietic system and has nine key tyrosine residues. c-Kit N705A/N938A-expressing Ba/F3 cells (with GNNK- isoform) has a N-to-A mutation at position 705 and 938, which results in reduced Cbl phosphorylation but, both mutations do not affect the activation of Src family kinases. The GNNK- isoform is the outcome of the c-Kit gene alternative splicing sites, resulting in different isoforms of the the tetrapeptide GNNK, and is the main transcript found in human malignancies. Together with the wild-type c-Kit stable Ba/F3 cells (

Biosafety:

I

Organism:

Mouse

Source Organ:

Marrow

Growth Properties:

Suspension

Morphology:

Round

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

Transfected with pMSCVpuro vector carrying mutant N705A/N938A human c-Kit construct

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow II medium available at abm

Preservation:

N/A

Quality Control:

1) Western blotting for presence of c-Kit; 2) PCR

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C